Structural consequences of dry heating on alpha-lactalbumin and beta-lactoglobulin at pH 6.5
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Analysis of bovine whey proteins in soybean dairy-like products by capillary electrophoresis.
The simultaneous separation of bovine whey proteins [alpha-lactalbumin and beta-lactoglobulin (A+B)] and soybean proteins was performed, for the first time, by capillary electrophoresis. Different experimental conditions were tested. The most suitable consisted of 0.050 M phosphate buffer (pH 8) with 1 M urea and 1.2 mg/ml methylhydroxyethylcellulose, UV detection at 280 nm, 15 kV applied volta...
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Aggregation of beta-lactoglobulin occurs mainly via intermolecular disulphide bond exchange. Upon heating, beta-lactoglobulin aggregated which increased with increasing pH. The presence of DTT led to more rapid aggregation and precipitation of beta-lactoglobulin. AlphaCrystallin prevented the aggregation of heat-stressed beta-lactoglobulin and was a more efficient chaperone at higher pH values....
متن کاملSelective separation of the major whey proteins using ion exchange membranes.
Synthetic microporous membranes with functional groups covalently attached were used to selectively separate beta-lactoglobulin, BSA, and alpha-lactalbumin from rennet whey. The selectivity and membrane performance of strong (quaternary ammonium) and weak (diethylamine) ion-exchange membranes were studied using breakthrough curves, measurement of binding capacity, and protein composition of the...
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Introduction & Objective: Ficin is a member of plant cystein proteases that is abundant in fig. This enzyme has many pharmacological and industrial uses. In the present study, the enzyme was purified by a simple procedure and its proteolytic activity was assayed on several plant and animal proteins. Materials & Methods: Ficin was extracted from unripe fig, precipitated by ammonium sulfate and...
متن کاملTransport of beta-lactoglobulin and alpha-lactalbumin in enterocyte-like Caco-2 cells.
The transport of [14C]-radiolabelled beta-lactoglobulin and alpha-lactalbumin through Caco-2 cell monolayers grown on permeable filters was studied in order to evaluate the different protein pathways through the intestinal epithelium. beta-Lactoglobulin or alpha-lactalbumin (0.25-3 mg/ml) was introduced on the apical side of the monolayer and both the transport and the release of labelled mater...
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